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Single-cell Sequencing technology (CAT#: STEM-MB-0074-WXH)

Introduction

Single-cell sequencing technology refers to the sequencing of single-cell genomes or transcriptomes to obtain genome, transcriptome or other multi-omics information, thereby revealing differences in cell populations and cell developmental lineage relationships. The information obtained by traditional sequencing methods generally comes from mixed cell samples, so the sequencing method often loses the heterogeneity information of cells. Single-cell sequencing technology combined with optimized NGS sequencing technology has great advantages in detecting heterogeneity between single cells, identifying rare cells, and drawing cell maps. Can help researchers study cell function and better understand cell-to-cell differentiation, cell lineage relationships, and disease evolution. Single-cell sequencing also facilitates the discovery of potential biomarkers and drug targets, as well as the analysis of rare cell types.




Applications

• Identifying new mutations in cancer cells
• Exploring the progressive epigenomic changes that occur during embryonic development
• Assess how a seemingly homogeneous population of cells expresses specific genes

Procedure

1. Isolation of single cells from cell populations.
2. Extracting, processing and amplifying the genetic material of each isolated cell.
3. Preparation of a sequencing library comprising the genetic substance of the isolated cells.
4. Sequence the library using a second-generation sequencer.