DNA Sequencing (CAT#: STEM-MB-0040-WXH)

Introduction

DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. <br />DNA sequencing is the process of determining the sequence of nucleic acids -- the order of nucleotides in DNA. It includes any method or technique used to determine the order of the four bases: adenine, guanine, cytosine, and thymine.<br />The principle of DNA sequencing is to use a DNA polymerase to extend the primer bound to the undetermined sequence template until a chain-terminating nucleotide is incorporated.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Disease diagnosis
• Gene therapy
• Genetic research
• Drug Trials and Pharmacogenomics
• Evolutionary biology
• Metagenomics
• Virology
• Forensic investigation

Procedure

1. DNA fragment amplification
2. Cyclic testing, forming DNA strands with terminal fluorescent labels
3. Gel electrophoresis

Notes

Customers provide sequencing information sheets, sequencing samples (including bacterial liquid, plasmids, PCR products) and sequencing primers.

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