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Molecular Cloning and Gene Vector Construction (CAT#: STEM-MB-0005-WXH)

Introduction

Molecular cloning is an essential process used in scientific research and discovery. With molecular cloning scientists can amplify and manipulate genes of interest and then insert them into plasmids for replication and protein expression. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.




Applications

• Genome organizationgene expression
• Production of recombinant proteins
• Transgenic organisms
• Gene therapy

Procedure

1.Choice of host organism and cloning vector
2.Preparation of vector DNA
3.Preparation of DNA to be cloned
4.Creation of recombinant DNA
5.Introduction of recombinant DNA into host organism
6.Selection of organisms containing recombinant DNA
7.Screening for clones with desired DNA inserts and biological properties

Notes

• Customer inform the main purpose and specific requirements.
• Customers provide source and background information of exogenous DNA and vectors.
• Customers provide electrophoresis photos of DNA.