MSCV Retrovirus Vector Construction/MSCV Retrovirus Packaging for gene delivery (CAT#: STEM-MB-0011-WXH)

Introduction

Recombinant murine stem cell virus (MSCV) vectors serve as efficient viral vector tools for achieving robust expression of genes of interest in embryonic stem (ES) cells, embryonal carcinoma (EC) cells and hematopoietic stem (HS) cells, along with several other mammalian cell lines. In this retroviral vector system, the presence of a strategically designed 5’LTR derived from the murine stem cell PCMV virus in the MSCV retroviral vector contributes to the transcriptional activation of target genes in pluripotent cell lines. This helps to overcome the restricted expression of target genes in ES and EC cells, typically observed with MMLV retroviral vectors.