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Analysis of Total Phenols by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1330-LGZ)
Introduction
Sensitivity: 0.73 μg/mL
Detection range: 0.73-150 μg/mL
Precision: inter-lot difference of 2.5%, intra-lot difference of 1.9%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 760 nm
Principle
Under basic conditions, phenolic substances reduce tungstate molybdenum acid to produce blue compounds. At the characteristic absorption peak of 760 nm, the color depth is proportional to the content of phenol. The total content of phenol can be calculated by colorimetric analysis.
Applications
It is suitable for the determination of total phenol content in plant tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: Plant tissue
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