Analysis of Citrate Synthase(CS) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1476-LGZ)

Citrate synthetase (CS) catalyzes acetyl coA and oxaloacetic acid to produce limonyl coA and further to produce coA. This reaction can produce yellow substance with characteristic light absorption value at 412 nm. The activity of CS enzyme can be detected by increasing the light absorption value

It is used to detect the activity of citrate synthase (CS) in animal and plant tissue and cell samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal and plant tissue and cell samples