Analysis of Glutaminase (GLS) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1467-LGZ)

Under the action of glutaminase, glutamine is decomposed to produce glutamic acid and ammonia, and glutamic acid is further oxidized and dehydrogenated to produce α-ketoglutaric acid under the catalysis of glutamate dehydrogenase. At the same time, NAD+ is reduced to produce NADH, which makes WST-8 orange in color under the action of hydrogen-delivering substances. Glutaminase activity can be measured by the change of absorbance value at 450 nm.

It is suitable for detecting the activity of glutaminase (GLS) in serum (plasma), animal and plant tissue samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, animal and plant tissue samples