Analysis of Sucrose by Fluorescence Microplate Reader (CAT#: STEM-MB-1511-LGZ)

Under acidic conditions, sucrose can be hydrolyzed into glucose by sucrose enzyme, which is catalyzed by glucose oxidase to produce hydrogen peroxide. Under the action of horseradish peroxidase, hydrogen peroxide can react with fluorescent probe to produce strong red fluorescent substance. The fluorescence intensity is linear with sucrose content within a certain range.

It is suitable for the determination of sucrose content in various plant tissues.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

• Sample Type: various plant tissues