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Analysis of Polyphenol Oxidase (PPO) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1423-LGZ)
Introduction
Precision: inter-lot difference of 8.699999999999999%, intra-lot difference of 4.6%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 405-415 nm
Principle
Polyphenol oxidase (PPO) can catalyze the oxidation of phenolic compounds to quinones. The latter has characteristic light absorption at 410 nm. The activity of PPO enzyme can be calculated by comparing with the increase of absorbance value.
Applications
Used to detect the activity of polyphenol oxidase (PPO) in plant tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: plant tissue samples
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