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Analysis of Cell Ferrous Iron by Enzyme-labeled Instrument (CAT#: STEM-MB-1462-LGZ)

Introduction

Sensitivity: 0.4 μmol/L<br />Detection range: 0.4 - 50 μmol/L<br />Precision: inter-lot difference of 1.5%, intra-lot difference of 1.5%<br />Detection equipment: Enzyme-labeled Instrument<br />Detection wavelength: 590 - 600 nm




Principle

Ferrous ions in the sample were combined with the probe, and the resulting material had a strong absorption peak at 593 nm wavelength, and its optical density was linearly correlated with the concentration of ferrous ions within a certain range.

Applications

Used to detect ferrous content in cell samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: cell samples