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Analysis of Glucose Oxidase (GOD) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1481-LGZ)
Introduction
Sensitivity: 0.006 U/L
Detection range: 0.006–0.1 U/L
Precision: inter-lot difference of 7%, intra-lot difference of 3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 540-560 nm
Principle
Under the action of glucose oxidase, glucose generates hydrogen peroxide, and hydrogen peroxide makes chromogenic substances chromogenic under the action of peroxidase. The activity of GOD is measured by the absorbance at 550 nm.
Applications
It is suitable for detecting the activity of glucose oxidase (GOD) in samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: animal tissues and cells
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For various laboratory needs
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