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Analysis of Phosphorus (Pi) by Enzyme-labeled Instrument (CAT#: STEM-MB-1410-LGZ)
Introduction
Sensitivity: 0.004 mmol/L
Detection range: 0.004-2.0 mmol/L
Precision: inter-lot difference of 3%, intra-lot difference of 2.1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 620 nm-690nm
Principle
Inorganic phosphorus reacts with molybdic acid to form phosphomolybdic acid. Under the action of reducing agent, phosphomolybdic acid is reduced to molybdenum blue. It has the maximum absorption peak at 660 nm, and the absorbance value is proportional to the concentration within a certain range.
Applications
Applicable to the detection of phosphorus content in serum, plasma and tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum, plasma and tissue samples
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