Analysis of Glycogen by Fluorescence Microplate Reader (CAT#: STEM-MB-1509-LGZ)

Glycogen produces glucose under the action of amylosidase, which is catalyzed by glucose oxidase to produce hydrogen peroxide. Under the action of peroxidase, hydrogen peroxide oxidizes the non-fluorescent probe into a fluorescent substance, and its fluorescence intensity is related to the content of glycogen.

It is suitable for the detection of glycogen content in liver and muscle tissue of animal.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

• Sample Type: liver and muscle tissue of animal