Analysis of Acid Phosphatase (ACP) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1335-LGZ)

Acid phosphatase can decompose phenylene disodium phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol reacts with 4-amino-antipyrine in alkaline solution and oxidizes to red quinone derivatives by potassium ferricyanide. The activity of enzyme can be measured according to the shade of red.

Used to detect the activity of acid phosphatase in animal serum (plasma), body fluid, tissue and cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal serum (plasma), body fluid, tissue and cells