Analysis of Free Fatty Acids (NEFA/FFA) by Fluorescence Microplate Reader (CAT#: STEM-MB-1506-LGZ)

Free fatty acids are produced by acyl synthetase to form acyl coA, which is produced by the oxidation of acyl oxidase to form hydrogen peroxide. The hydrogen peroxide reacts in the presence of enzyme and probe, and the fluorescence intensity of the produced fluorescent substance at the excitation wavelength of 535 nm and the emission wavelength of 590 nm is proportional to the concentration of free fatty acids.

Used to detect the content of free fatty acids in serum, plasma and animal tissue samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma and animal tissue samples