Analysis of Aldehyde Dehydrogenase (ALDH) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1466-LGZ)

The substrate converts NAD+ into NADH under the action of acetaldehyde dehydrogenase, and NADH makes WST-8 orange color under the action of electron coupling agent. The activity of acetaldehyde dehydrogenase can be measured by measuring the change of absorbance value at 450 nm.

It was used to detect the activity of acetaldehyde dehydrogenase (ALDH) in animal tissue, serum (plasma) and other samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue, serum (plasma) and other samples