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Analysis of Na+k+-ATPase Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1430-LGZ)
Introduction
Sensitivity: 0.11 μmol Pi/mL/hour
Detection range: 0.42-4.99 μmol Pi/mL/hour
Precision: inter-lot difference of 2.2%, intra-lot difference of 2.2%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 640-680 nm
Principle
Na+K+ -ATPase decomposed ATP to produce ADP and phosphorus. The activity of Na+K+ -ATPase was calculated by determining the content of phosphorus.
Applications
Used to detect Na+K+ -ATPase activity in serum (plasma) and animal tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum (plasma) and animal tissue samples
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For various laboratory needs
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