Analysis of Aspartate Aminotransferase (AST/GOT) Activity by Fluorescence Microplate Reader (CAT#: STEM-MB-1514-LGZ)

Glutamic oxaloacetic acid catalyzed substrate reaction to oxaloacetic acid, oxaloacetic acid decarboxylated to pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to fluoresce. The increase of fluorescence intensity in the system was proportional to AST activity.

It was used to detect the activity of AST in serum, plasma, animal tissue, cell and cell supernatant samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, animal tissue, cell and cell supernatant samples