Analysis of Dipeptidyl Peptidase IV (DPP4) Activity by Fluorescence Microplate Reader (CAT#: STEM-MB-1513-LGZ)

Dipeptidyl peptidase 4, also known as CD26, is a serine protease that can break down the peptide bond of the second proline or alanine residue at the N end of the peptide chain. In vivo, dipeptidyl peptidase can rapidly break down enterocapsin, which can stabilize insulin levels and promote the reduction of blood sugar levels in the organism. The detection principle of this experiment is that dipeptidyl peptidase can decompose the substrate to release the fluorescent substance AMC, while adding dipeptidyl peptidase inhibitor can inhibit the activity of the enzyme. The activity of the enzyme can be calculated by the difference of fluorescence value before and after inhibition.

It is used to detect the enzyme activity of dipeptidyl peptidase (DPP4) in serum, plasma, animal tissue and cell samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, animal tissue and cell samples