Analysis of Catalase (CAT) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1497-LGZ)

Catalase catalyzes the decomposition of H2O2 into oxygen and water. Ammonium molybdate can quickly stop the decomposition of H2O2 by CAT, and the remaining H2O2 reacts with ammonium molybdate to produce a light yellow complex. The activity of CAT can be calculated by measuring its production quantity at wavelength 405 nm.

It is suitable for the detection of catalase activity in serum, plasma, cultured cells, culture medium and animal and plant tissue samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, cultured cells, culture medium and animal and plant tissue samples