Analysis of Mitochondrial Complex I (NADH-CoQ Reductase) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1477-LGZ)

Mitochondrial respiratory chain complex I catalyzes the reaction of NADH with panquinone substrate to generate NAD+ and reduced panquinone. The activity of NADH can be reflected by the determination of the absorbance decline rate at 340 nm. The specific inhibitor rotenone can inhibit this enzyme, and the total enzyme activity minus the inhibitory activity of the inhibitor can reflect the specific enzyme activity.

To detect the activity of mitochondrial respiratory chain complex I(NADh-Coenzyme Q reductase) in animal tissue samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue samples