Analysis of Acetyl-CoA by Enzyme-labeled Instrument (CAT#: STEM-MB-1475-LGZ)

Malic acid and NAD+ are treated by malate dehydrogenase to produce oxaloacetic acid and NADH, and acetyl-coA and oxaloacetic acid are treated by citrate synthetase to produce coA and citrate. Under the coupling of malate dehydrogenase and citrate synthetase, the content of acetyl-coA was determined by absorbance change at 340 nm.

This method was used to detect the Acetyl-CoA content in animal tissue samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue samples