Analysis of Lactate Dehydrogenase (LDH) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1490-LGZ)

Using coenzyme I as the hydrogen transfer body, LDH catalyzes lactic acid to produce pyruvate, which reacts with 2, 4-dinitrophenylhydrazine to produce dinitrophenylhydrazone pyruvate. The latter is brown-red in alkaline solution, and the shade of the color is proportional to the concentration of pyruvate. The activity of LDH can be calculated by measuring OD value.

It is suitable for the detection of LDH activity in serum, plasma, pleural fluid, tissue, cell and other samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, pleural fluid, tissue, cell and other samples