Analysis of CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1352-LGZ)

Superoxide anion radical (O2.-) is produced through the xanthine and xanthine oxidase reaction system, which oxidizes hydroxylamine to form nitrite and produces purplish red compounds under the action of chromogenic agents. When SOD was added into the reaction system, the disproportionation of superoxide anion radical was catalyzed, and the formation of nitrite was reduced, and then the purplish red was lighter. SOD activity was calculated according to the absorbance difference between the sample tube and the charge. The cells of higher organisms contain Cu-zn-SOD (Cu-Zn-SOD) and Mn-SOD (Mn-SOD). At the same time, the activity of Mn-SOD is lost in the sample pretreatment process, but the activity of Cu-Zn-SOD remains unchanged.

Used to detect the activity of T-SOD, CuZn-SOD and Mn-SOD in serum (plasma), urine, cells and cell supernatant, various animal and plant tissues.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum (plasma), urine, cells and cell supernatant, various animal and plant tissues