Analysis of Erythrocyte by Flow Cytometry (CAT#: STEM-CBT-0073-WXH)

Introduction

1.The detection and quantification of fetal red cells in maternal blood. <br /> The use of flow cytometry for the detection of fetal cells is much more objective, reproducible, and sensitive than the Kleihauer-Betke test <br />2.Reticulocyte counts <br /> Reticulocyte counts are based on identification of residual ribosomes and RNA in immature nonnucleated red blood cells (RBCs). This method provides excellent discrimination between reticulocytes and mature RBCs, with greater precision, sensitivity, and reproducibility than the traditional method. A reticulocyte maturity index has been used clinically to assess bone marrow engraftment and erythropoietic activity and to help classify anemias.<br />3.Blood bank<br /> Flow cytometry can be used as a complementary or replacement test for red cell immunology, including RBC-bound immunoglobulins and red cell antigens. Flow cytometry has been used to accurately identify and phenotype the recipient’s red cells. <br />4.Evaluating RBC Shape<br />Flow cytometry can detect any shape alterations in RBCs to identify disease mechanisms and the likelihood of a patient developing a particular condition. <br />5.assess alterations in RBC rheology<br />Red blood cell (RBC) rheology is altered in different diseases, including acute conditions such as patients in intensive care units (ICU) with sepsis or with an inflammatory reaction due to postoperative states or intracerebral haemorrhage, or chronic conditions such as diabetes mellitus or terminal renal failure.