Total Bacterial Counts by Flow Cytometry (CAT#: STEM-CBT-0078-WXH)

Introduction

The total bacteria count (TBC) of a substance is a quantitative estimate of the number of microorganisms present in a sample, which is an important and straightforward indicator to evaluate the biological quality of the targeted habitat. Due to the rapid speed and automated sample handling, FCM has been widely used in the basic research of microbiology, routine monitoring of food safety, and quality control of drinking water. A good discrimination of the small sizes of bacterial cells from abiotic particles and background signal is the prerequisite for the accurate counting of bacterial cells using FCM.

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Principle Applications Procedure Materials Notes Related Products

Principle

Benefiting from the development of intercalating dyes, bright fluorescence can be obtained for individual bacterial cells upon nucleic acid staining, which enables efficient detection by the FCM

Applications

• Basic research of microbiology.
• Routine monitoring of food safety.
• Quality control of drinking water.

Procedure

1.Sample pretreatment.
2.Fluorescence staining
3.Detection by flow cytometry

Materials

Among all the DNA stains, PicoGreen,
SYTO dyes, SYBR dyes, SYTOX dyes, and acridine orange have been widely used to stain the genomic DNA of bacterial cells for microbial quantification.

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