Analysis of Cholinesterase (CHE) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1356-LGZ)

Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine, which is not hydrolyzed by cholinesterase, reacts with basic hydroxylamine to generate acetylhydroxylamine, which reacts to form brown red hydroxamic acid iron complex in acidic solution. The color depth is proportional to the amount of residual acetylcholine, which can be quantified by colorimetric method, so as to calculate the activity of cholinesterase.

Used to detect cholinesterase activity in whole blood, serum (plasma), tissue and cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: whole blood, serum (plasma), tissue and cells