Analysis of Pyruvate Dehydrogenase (PDH) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1484-LGZ)

Under the action of CoA, pyruvate can be catalytic decarboxylate by PDH, and NAD+ can be reduced to NADH, which reacts with the color developer to form colored substance under the action of electron transporter. The maximum absorption of the substance is at 450 nm, and the activity of PDH can be determined by calculating the change value in unit time.

Used to detect the activity of pyruvate dehydrogenase (PDH) in serum, plasma, urine, animal tissue and cell samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, urine, animal tissue and cell samples