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Analysis of Choline Acetyltransferase (ChAT) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1354-LGZ)
Introduction
Sensitivity: 1.21U/g
Detection range: 1.21-40 U/g
Precision: inter-lot difference of 9.199999999999999%, intra-lot difference of 5%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 324 nm
Principle
Acetylcholintransferase (ChAT) takes acetyl-coA and choline as substrate to produce CoA (CoA). CoA combines with color developing agent 4, 4-dithiopyridine. Absorbance is measured at 324nm to calculate the activity of ChAT.
Applications
Used to detect ChAT activity in various animal tissues.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: various animal tissues
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For various laboratory needs
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