Analysis of High-density Lipoprotein Cholesterol (HDL-C) by Enzyme-labeled Instrument (CAT#: STEM-MB-1378-LGZ)

CM, VLDL and LDL coagulated to form polymers and were masked by polymers in the polyanionic environment. High density lipoprotein (HDL) formed soluble complex under the action of surfactants, so that HDL-C could react directly with enzyme reagents [containing cholesterol esterase (CE), cholesterol oxidase (CO), etc.]. Hydrogen peroxide is produced. When hydrogen peroxide is present in 4-amino-antipyrline (4-AA) and phenol (TOOS), it is catalyzed by oxidase (POD) to generate red quinone compound benzoquinimide phenazone. The color depth is proportional to the content of HDL-C.

It is suitable for the determination of high density lipoprotein cholesterol in serum (plasma) samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma