Analysis of Uric Acid (UA) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1312-LGZ)

Introduction

Sensitivity: 0.58 mg/L<br />Detection range: 0.58-100 mg/L<br />Precision: inter-lot difference of 2.6%, intra-lot difference of 1.8%<br />Detection equipment: Ultraviolet-visible Spectrophotometer<br />Detection wavelength: 690 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Phosphotungstic acid reduction method uses uric acid (UA) in protein-free filtrate to reduce phosphotungstic acid to produce tungstic blue, allantoin and carbon dioxide under alkaline conditions. The color shade is proportional to the concentration of uric acid.

Applications

Used to detect the content of uric acid in serum, plasma and urine samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, urine

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