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Analysis of Cell Total Iron by Enzyme-labeled Instrument (CAT#: STEM-MB-1385-LGZ)
Introduction
Sensitivity: 0.4 μmol/L
Detection range: 0.4 - 50 μmol/L
Precision: inter-lot difference of 1.5%, intra-lot difference of 1.3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 590 - 600 nm
Principle
Under the action of the reducing agent, the iron ions in the sample were reduced to ferrous ions, which combined with the probe. The resulting material had a strong absorption peak at 593 nm wavelength, and its optical density was linearly related to the concentration of iron ions within a certain range.
Applications
It is suitable for the determination of total iron ion content in cell samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: cell samples
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