Analysis of Aspartate Aminotransferase (AST/GOT) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1493-LGZ)

Glutamic oxaloacetic acid (AST) catalyzes the amino conversion reaction between aspartate and α-ketoglutaric acid to produce glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate to pyruvate in the reaction process. Pyruvate reacts with phenylhydrazine to form phenylhydrazone, which is reddish brown in alkaline conditions.

It is suitable for the detection of AST activity in serum (plasma), animal tissues, cells and cell supernatant.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum (plasma), animal tissues, cells and cell supernatant