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Analysis of Lipid Peroxide (LPO) by Enzyme-labeled Instrument (CAT#: STEM-MB-1495-LGZ)
Introduction
Sensitivity: 0.70 μmol/L
Detection range: 0.70-80 μmol/L
Precision: inter-lot difference of 3.5%, intra-lot difference of 3.1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 580-590 nm
Principle
Under acidic conditions at 45℃, lipid peroxides combine with the chromogenic agent to produce stable chromogenic group with maximum absorption peak at 586 nm and absorbance value is proportional to concentration within a certain range.
Applications
Applicable to the detection of lipid peroxide content in serum (plasma), urine and tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum, plasma, urine and tissue samples
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For various laboratory needs
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