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Analysis of α-Amylase Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1417-LGZ)
Introduction
Sensitivity: 0.97 U/g
Detection range: 0.97-34.74 U/g
Precision: inter-lot difference of 3.9%, intra-lot difference of 3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 535-540 nm
Principle
The reducing sugar reacted with 3, 5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. The β-amylase was passivated by taking advantage of its heat-resistant property, and then the activity of α-amylase was measured.
Applications
It can be used to detect α-amylase activity in plant and animal tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: plant and animal tissue samples
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