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SILAC for quantitative proteomics (CAT#: STEM-MB-0085-WXH)
Introduction
Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. It is a popular method for quantitative proteomics.
The principle of SILAC is based on metabolically incorporating stable isotope labeled amino acids into the entire proteome.
Applications
• Study of cell signaling, post translation modifications, protein–protein interaction and regulation of gene expression.
• Globle study of secreted proteins and secretory pathways.
• Globle study of secreted proteins and secretory pathways.
Procedure
1. Cell culture markers (cultured in light and heavy amino acid medium respectively)
2. Protein purification
3. Mixed protein samples
4. Protein reduction and digestion
5. Detection by liquid chromatography-mass spectrometry (LC-MS)
6. Bioinformatics analysis
2. Protein purification
3. Mixed protein samples
4. Protein reduction and digestion
5. Detection by liquid chromatography-mass spectrometry (LC-MS)
6. Bioinformatics analysis
Notes
The customer provides or informs the cell line used in the experiment.
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Wide range of applications
For various laboratory needs
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