PRM Quantitative Proteomics to detect target protein with high resolution (CAT#: STEM-MB-0090-WXH)

Introduction

Parallel Reaction Monitoring (PRM) is a high-resolution, high-precision mass spectrometry-based ion monitoring technique that enables selective detection of target proteins and target peptides (e.g., peptides undergoing post-translational modifications) for quantification of target proteins/peptides.<br />It is characterized by high specificity, sensitivity and accuracy, and is capable of quantitative protein analysis in batch.<br />The principle is the relative/absolute quantification of proteins using secondary fragment ions. The PRM technique based on the electrostatic field orbital trap Obitrap/Time of Flight TOF mass analyzer is capable of highly specific selection of peptide ions with a preset mass-to-charge ratio and fragmentation, detection of all fragmentation information generated by the peptide, calculation of the intensity of the ions, and thus quantification of the peptide.