Measurement of Magnesium by Ion Selective Electrode (CAT#: STEM-ACT-0113-WXH)

Introduction

Magnesium is a component of bone; in fact 60% of the body's magnesium is stored in bone. It is also involved with the activity of bone-building cells and the parathyroid hormone, which regulates calcium levels.<br />Magnesium (Mg++) ions serve various physiological functions in living cells. Its primary role is as a co-factor in many enzymatic reactions, especially those involved in energy production and ATP utilization.

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Principle Applications Procedure Materials Notes Related Products

Principle

An ideal ion-selective electrode (I.S.E.) consists of a thin membrane across which only the intended ion can be transported. The transport of ions from a high conc. to a low one through a selective binding with some sites within the membrane creates a potential difference.

Applications

• Used for the potentiometric determination of several anions (fluoride, chloride, bromide, iodide ion) and cations (sodium, potassium, calcium ion).
• Used for endpoint detection in potentiometric titration.
• In biochemistry, ion selective electrodes are used for the determination of enzymes and substances which interact with enzymes.

Procedure

1. Preparation of Standards
2. Instrument Setup and Operation
3. Analysis of sample

Materials

Ion Selective Electrode

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