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Measurement of Copper by Ion Selective Electrode (CAT#: STEM-ACT-0105-WXH)

Introduction

Copper is a chemical element with the symbol Cu and atomic number 29. It is a soft, malleable, and ductile metal with very high thermal and electrical conductivity. <br />Copper ions are essential for cellular physiology, yet toxic. As cofactors for a number of enzymes, copper ions are required for cellular processes such as respiration, neural transmission, tissue maturation, defense against oxidative stress and iron metabolism. A sufficient intracellular copper ion level must be maintained in order to establish these important processes.




Principle

An ideal ion-selective electrode (I.S.E.) consists of a thin membrane across which only the intended ion can be transported. The transport of ions from a high conc. to a low one through a selective binding with some sites within the membrane creates a potential difference.

Applications

• Used for the potentiometric determination of several anions (fluoride, chloride, bromide, iodide ion) and cations (sodium, potassium, calcium ion).
• Used for endpoint detection in potentiometric titration.
• In biochemistry, ion selective electrodes are used for the determination of enzymes and substances which interact with enzymes.

Procedure

1. Preparation of Standards
2. Instrument Setup and Operation
3. Analysis of sample

Materials

Ion Selective Electrode