Measurement of TAN (total ammonia nitrogen) by Ion Selective Electrode (CAT#: STEM-ACT-0116-WXH)

Introduction

TAN (total ammonia nitrogen) is the total amount of nitrogen in the forms of NH3 and NH4+ in water. In aquaculture, TAN concentration must be less than 0.5 mg/L.

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Principle Applications Procedure Materials Notes Related Products

Principle

An ideal ion-selective electrode (I.S.E.) consists of a thin membrane across which only the intended ion can be transported. The transport of ions from a high conc. to a low one through a selective binding with some sites within the membrane creates a potential difference.

Applications

• Used for the potentiometric determination of several anions (fluoride, chloride, bromide, iodide ion) and cations (sodium, potassium, calcium ion).
• Used for endpoint detection in potentiometric titration.
• In biochemistry, ion selective electrodes are used for the determination of enzymes and substances which interact with enzymes.

Procedure

1. Preparation of Standards
2. Instrument Setup and Operation
3. Analysis of sample

Materials

Ion Selective Electrode

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