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Measurement of potassium by Ion Selective Electrode (CAT#: STEM-ACT-0100-WXH)

Introduction

Potassium is an essential mineral that is needed by all tissues in the body. It is sometimes referred to as an electrolyte because it carries a small electrical charge that activates various cell and nerve functions. Potassium is found naturally in many foods and as a supplement.




Principle

An ideal ion-selective electrode (I.S.E.) consists of a thin membrane across which only the intended ion can be transported. The transport of ions from a high conc. to a low one through a selective binding with some sites within the membrane creates a potential difference.

Applications

• Used for the potentiometric determination of several anions (fluoride, chloride, bromide, iodide ion) and cations (sodium, potassium, calcium ion).
• Used for endpoint detection in potentiometric titration.
• In biochemistry, ion selective electrodes are used for the determination of enzymes and substances which interact with enzymes.

Procedure

1. Preparation of Standards
2. Instrument Setup and Operation
3. Analysis of sample

Materials

Ion Selective Electrode