Ion Selective Electrode Analysis (CAT#: STEM-ACT-0096-WXH)

Introduction

Ion selective electrode (ISE) is an analytical technique used to determine the activity of ions in aqueous solution by measuring the electrical potential. ISE has many advantages compared to other techniques, including: It is relatively inexpensive and easy to operate. It has wide concentration measurement range. As it measure the activity, instead of concentration, it is particularly useful in biological/medical application. It is a real-time measurement, which means it can monitor the change of activity of ion with time. It can determine both positively and negatively charged ions.

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Principle Applications Procedure Materials Notes Related Products

Principle

An ideal ion-selective electrode (I.S.E.) consists of a thin membrane across which only the intended ion can be transported. The transport of ions from a high conc. to a low one through a selective binding with some sites within the membrane creates a potential difference.

Applications

• Used for the potentiometric determination of several anions (fluoride, chloride, bromide, iodide ion) and cations (sodium, potassium, calcium ion).
• Used for endpoint detection in potentiometric titration.
• In biochemistry, ion selective electrodes are used for the determination of enzymes and substances which interact with enzymes.

Procedure

1. Preparation of Standards
2. Instrument Setup and Operation
3. Analysis of sample

Materials

Ion Selective Electrode

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