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Chromatin Immunoprecipitation Sequecing (ChIP-seq) (CAT#: STEM-MB-0043-WXH)
Introduction
Chromatin immunoprecipitation sequecing (ChIP-seq), is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest.
The main advantages of ChIP-seq are the excellent base pair resolution and the reduced number of samples required.
Applications
• Determining what kind of histone modification occurs at a specific position in the DNA strand
• Detection of the precise location of binding sites of RNA polymerase II and other trans-factors on the genome
• Investigating the relationship between histone covalent modification and gene expression
• CTCF Transcription Factor Research
• Examination of the interactions between proteins and nucleic acids
• Detection of the precise location of binding sites of RNA polymerase II and other trans-factors on the genome
• Investigating the relationship between histone covalent modification and gene expression
• CTCF Transcription Factor Research
• Examination of the interactions between proteins and nucleic acids
Procedure
1. DNA enrichment quality inspection
2. Library construction and sequencing
3. Data filtering quality control
4. Reference genome comparison
5. Data Analysis
2. Library construction and sequencing
3. Data filtering quality control
4. Reference genome comparison
5. Data Analysis
Notes
Customers provide experimental samples.
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For various laboratory needs
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