Methylated DNA Immunoprecipitation Sequencing (MeDIP-Seq) to analyze DNA Methylation (CAT#: STEM-MB-0050-WXH)

Introduction

Methylated DNA Immunoprecipitation Sequencing (MeDIP-Seq) is a genome-wide methylation detection technology based on the principle of antibody enrichment, which uses methylated DNA immunoprecipitation technology to specifically enrich for methylated DNA fragments on the genome by 5'-methylcytosine antibodies. High-throughput sequencing then allows for high-precision studies of CpG-dense hypermethylated regions at the whole-genome level. Researchers can use MeDIP-Seq technology to quickly and efficiently search for methylated regions on the genome and thus compare differences in DNA methylation modification patterns between different cell, tissue or disease samples.

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Applications

• Detect DNA methylation signal enriched regions in disease samples and infer the set of candidate genes whose expression is suppressed in the samples.
• Detect the dynamic DNA methylation profiles at the focal site during different periods of disease development, and screen for epigenetic markers to help define the extent of disease development.
• Compare the differences in signal and location distribution of DNA methylation regions in disease samples and normal samples, screen for disease-specific DNA methylation regions, observe genes around these regions, and narrow down disease-associated candidate genes.

Procedure

1.Sample quality control
2.Library construction
3.Antibody enrichment of methylated fragments
4.On-site detection
5.Information analysis

Notes

Customer provides tissue, cell, DNA and other samples and related information

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