small RNA sequencing (CAT#: STEM-MB-0069-WXH)

Introduction

Small noncoding RNAs act in gene silencing and post-transcriptional regulation of gene expression. Small RNA sequencing (RNA-Seq) is a technique to isolate and sequence small RNA species). Small RNA-Seq can query thousands of small RNA and miRNA sequences with unprecedented sensitivity and dynamic range.<br />Small RNAs, including microRNAs, siRNAs, and piRNAs, are non-coding RNAs. Small RNA sequencing generally collects 18-40nt fragments in samples for sequencing analysis, studies all known small RNAs in a specific tissue in a specific state, and can also discover new small RNAs and predict their target genes.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Applications

• Understand how post-transcriptional regulation contributes to phenotype
• Identify novel biomarkers
• Capture the complete range of small RNA and miRNA species
• Cell Differentiation and Development Studies
• Regulatory Mechanism Research
• Disease marker search
• Disease Molecular Diagnosis
• Gene drug development

Procedure

1.Purification
2.Library Preparation and Amplification
3.Sequencing
4.Data analysis and storage

Notes

Customers provide total RNA, cells, or tissue samples

Other recommended products

SNP Genotyping (CAT#: STEM-MB-0055-WXH)
Reduced-Representation Sequencing (RRS-seq) (CAT#: STEM-MB-0054-WXH)
Genotyping by Sequencing (GBS) (CAT#: STEM-MB-0056-WXH)
DNA affinity purification sequencing (DAP-seq) to identify TFBS (CAT#: STEM-MB-0061-WXH)
Targeted region sequencing (TRS-seq） (CAT#: STEM-MB-0065-WXH)
LncRNA Sequencing (CAT#: STEM-MB-0044-WXH)
Low-coverage whole-genome sequencing (LcWGS-seq) (CAT#: STEM-MB-0049-WXH)
RNA Immunoprecipitation Sequencing (RIP-seq) (CAT#: STEM-MB-0071-WXH)