Analysis of Total Superoxide Dismutase (T-SOD) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1314-LGZ)

The superoxide anion free radical (O2.-) is generated through the xanthine and xanthine oxidase reaction system, which oxidizes hydroxylamine to form nitrite, and produces a purple compound under the action of the chromogenic agent. When SOD is added to the reaction system, it catalyzes the disproportionation reaction of superoxide anion radicals, which reduces the formed nitrite, and the resulting purple color becomes lighter. According to the absorbance difference in the sample tube and the control tube, the activity of SOD is calculated.

It is suitable for detecting T-SOD activity in serum (plasma), urine, cells and cell supernatant, and various animal and plant tissues.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: Serum (plasma), urinSerum (plasma), urine, cells and cell supernatant, various animal and plant tissues