Analysis of Total Oxidant Status (TOS) by Enzyme-labeled Instrument (CAT#: STEM-MB-1449-LGZ)

Name: Analysis of Total Oxidant Status (TOS) by Enzyme-labeled Instrument
SKU: STEM-MB-1449-LGZ
Rating: 5 (1 reviews)

Introduction

Sensitivity: 2.5 μmol H2O2 Equiv./L
Detection range: 2.5-100 μmol H2O2 Equiv./L
Precision: inter-lot difference of 3.5%, intra-lot difference of 2.3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 580-590 nm

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Principle Applications Procedure Materials

Principle

Under acidic conditions, the oxidizing material in the sample oxidizes Fe2+ to Fe3+, which is highly combined with xylenol orange to produce a blue-purple complex. When the pH of the solution is 2-3, the maximum absorption wavelength is around 590 nm, and the depth of color is proportional to the content of oxide in a certain concentration and a certain time, so as to indirectly measure the total oxidation capacity of the sample.

Applications

It is suitable for the detection of total oxidation status in tissue samples, serum and other liquid samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.