Analysis of BCA Protein by Enzyme-labeled Instrument (CAT#: STEM-MB-1370-LGZ)

The protein reduces the bivalent copper ion to the monovalent copper ion under alkaline conditions, which forms a purple complex with the BCA molecule. The complex can be detected at 540-590 nm wavelength, and the color of the reactant has a linear relationship with the protein concentration in a certain range.

It is suitable for the detection of protein content in serum, plasma, culture medium, animal and plant tissue and cultured cell samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, culture medium, animal and plant tissue and cultured cell samples