Analysis of Glucose-6-phosphate (G6P) by Enzyme-labeled Instrument (CAT#: STEM-MB-1432-LGZ)

Glucose-6-phosphate (G6P) is oxidized to form 6-phosphogluconolactone in the presence of glucose-6-phosphate dehydrogenase. In this reaction, NADP+ is reduced to NADPH. The resulting NADPH reduces WST-8 to orange-yellow formazan under the action of the electron-coupled reagent 1-mPMS. The maximum absorption peak is around 450 nm. The amount of formazan produced in the reaction system is directly proportional to the amount of total G6P in the sample.

It is suitable for the determination of glucose-6-phosphate in animal tissue samples and serum (plasma) and other liquid samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue samples and serum (plasma) and other liquid samples