Analysis of Pyruvate Kinase (PK) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1483-LGZ)

In the presence of adenosine diphosphate (ADP), pyruvate kinase (PK) catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, which is converted to lactic acid by LDH, and NAD+ by NADH. The activity of pyruvate kinase was calculated by detection of the change of absorbance value at 340 nm.

It is suitable for detecting pyruvate kinase (PK) activity in serum (plasma), animal tissues and cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum (plasma), animal tissues and cells